Functional characterization and expression patterns of PnATX genes under different abiotic stress treatments in Populus.
Identifieur interne : 000388 ( Main/Exploration ); précédent : 000387; suivant : 000389Functional characterization and expression patterns of PnATX genes under different abiotic stress treatments in Populus.
Auteurs : Zhiru Xu [République populaire de Chine] ; Jiahuan Huang [République populaire de Chine] ; Chunpu Qu [République populaire de Chine] ; Ruhui Chang [République populaire de Chine] ; Jinyuan Chen [République populaire de Chine] ; Qi Wang [République populaire de Chine] ; Qi Xi [République populaire de Chine] ; Yang Song [République populaire de Chine] ; Qi Sun [République populaire de Chine] ; Chuanping Yang [République populaire de Chine] ; Guanjun Liu [République populaire de Chine]Source :
- Tree physiology [ 1758-4469 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Proteins.
- genetics : Arabidopsis, Plants, Genetically Modified, Populus, Stress, Physiological.
- Gene Expression Regulation, Plant, Phylogeny.
Abstract
The copper chaperone ATX1 has been investigated previously in the herbaceous plants Arabidopsis and rice. However, the molecular mechanisms of ATX1 underlying copper transport and functional characteristics in the woody plant Populus are poorly understood. In this study, PnATX1 and PnATX2 of Populus simonii × P. nigra were identified and characterized. Sequence analysis showed that PnATXs contained the metal-binding motif MXCXXC in the N-terminus and a lysine-rich region. Phylogenetic analysis of ATX protein sequences revealed that PnATXs were clustered in the same group as AtATX1. PnATX proteins were localized in the cytoplasm and nucleus. Tissue-specific expression analysis showed that PnATX1 and PnATX2 were expressed in all analyzed tissues and, in particular, expressed to a higher relative expression level in young leaves. Quantitative real-time PCR analysis indicated that each PnATX gene was differentially expressed in different tissues under treatments with copper, zinc, iron, jasmonate and salicylic acid (SA). The copper-response element GTAC, methyl jasmonate and salicylic acid responsiveness elements and other cis-acting elements were identified in the PnATX1 and PnATX2 promoters. Expression of β-glucuronidase driven by the PnATX1 promoter was observed in the apical meristem of 7-day-old Arabidopsis transgenic seedlings, and the signal strength was not influenced by deficient or excessive copper conditions. Both PnATX1 and PnATX2 functionally rescued the defective phenotypes of yeast atx1Δ and sod1Δ strains. Under copper excess and deficiency conditions, transgenic Arabidopsis atx1 mutants harboring 35S::PnATX constructs exhibited root length and fresh weight similar to those of the wild type and higher than those of Arabidopsis atx1 mutants. Superoxide dismutase activity decreased in transgenic lines compared with that of atx1 mutants, whereas peroxidase and catalase activities increased significantly under excess copper. The results provide a basis for elucidating the role of Populus PnATX genes in copper homeostasis.
DOI: 10.1093/treephys/tpaa008
PubMed: 32031640
Affiliations:
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wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Jiahuan" sort="Huang, Jiahuan" uniqKey="Huang J" first="Jiahuan" last="Huang">Jiahuan Huang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation></author><author><name sortKey="Qu, Chunpu" sort="Qu, Chunpu" uniqKey="Qu C" first="Chunpu" last="Qu">Chunpu Qu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation></author><author><name sortKey="Chang, Ruhui" sort="Chang, Ruhui" uniqKey="Chang R" first="Ruhui" last="Chang">Ruhui Chang</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Jinyuan" sort="Chen, Jinyuan" uniqKey="Chen J" first="Jinyuan" last="Chen">Jinyuan Chen</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Qi" sort="Wang, Qi" uniqKey="Wang Q" first="Qi" last="Wang">Qi Wang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation></author><author><name sortKey="Xi, Qi" sort="Xi, Qi" uniqKey="Xi Q" first="Qi" last="Xi">Qi Xi</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation></author><author><name sortKey="Song, Yang" sort="Song, Yang" uniqKey="Song Y" first="Yang" last="Song">Yang Song</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Qi" sort="Sun, Qi" uniqKey="Sun Q" first="Qi" last="Sun">Qi Sun</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Chuanping" sort="Yang, Chuanping" uniqKey="Yang C" first="Chuanping" last="Yang">Chuanping Yang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Guanjun" sort="Liu, Guanjun" uniqKey="Liu G" first="Guanjun" last="Liu">Guanjun Liu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Forestry, Northeast Forestry University, Harbin, 150040, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Forestry, Northeast Forestry University, Harbin, 150040</wicri:regionArea><wicri:noRegion>150040</wicri:noRegion></affiliation></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (genetics)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plants, Genetically Modified (genetics)</term><term>Populus (genetics)</term><term>Stress, Physiological (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arabidopsis (génétique)</term><term>Phylogenèse (MeSH)</term><term>Populus (génétique)</term><term>Protéines végétales (génétique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Stress physiologique (génétique)</term><term>Végétaux génétiquement modifiés (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Plants, Genetically Modified</term><term>Populus</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arabidopsis</term><term>Populus</term><term>Protéines végétales</term><term>Stress physiologique</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Phylogenèse</term><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The copper chaperone ATX1 has been investigated previously in the herbaceous plants Arabidopsis and rice. However, the molecular mechanisms of ATX1 underlying copper transport and functional characteristics in the woody plant Populus are poorly understood. In this study, PnATX1 and PnATX2 of Populus simonii × P. nigra were identified and characterized. Sequence analysis showed that PnATXs contained the metal-binding motif MXCXXC in the N-terminus and a lysine-rich region. Phylogenetic analysis of ATX protein sequences revealed that PnATXs were clustered in the same group as AtATX1. PnATX proteins were localized in the cytoplasm and nucleus. Tissue-specific expression analysis showed that PnATX1 and PnATX2 were expressed in all analyzed tissues and, in particular, expressed to a higher relative expression level in young leaves. Quantitative real-time PCR analysis indicated that each PnATX gene was differentially expressed in different tissues under treatments with copper, zinc, iron, jasmonate and salicylic acid (SA). The copper-response element GTAC, methyl jasmonate and salicylic acid responsiveness elements and other cis-acting elements were identified in the PnATX1 and PnATX2 promoters. Expression of β-glucuronidase driven by the PnATX1 promoter was observed in the apical meristem of 7-day-old Arabidopsis transgenic seedlings, and the signal strength was not influenced by deficient or excessive copper conditions. Both PnATX1 and PnATX2 functionally rescued the defective phenotypes of yeast atx1Δ and sod1Δ strains. Under copper excess and deficiency conditions, transgenic Arabidopsis atx1 mutants harboring 35S::PnATX constructs exhibited root length and fresh weight similar to those of the wild type and higher than those of Arabidopsis atx1 mutants. Superoxide dismutase activity decreased in transgenic lines compared with that of atx1 mutants, whereas peroxidase and catalase activities increased significantly under excess copper. The results provide a basis for elucidating the role of Populus PnATX genes in copper homeostasis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">32031640</PMID><DateCompleted><Year>2020</Year><Month>10</Month><Day>01</Day></DateCompleted><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><Volume>40</Volume><Issue>4</Issue><PubDate><Year>2020</Year><Month>04</Month><Day>08</Day></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Functional characterization and expression patterns of PnATX genes under different abiotic stress treatments in Populus.</ArticleTitle><Pagination><MedlinePgn>520-537</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpaa008</ELocationID><Abstract><AbstractText>The copper chaperone ATX1 has been investigated previously in the herbaceous plants Arabidopsis and rice. However, the molecular mechanisms of ATX1 underlying copper transport and functional characteristics in the woody plant Populus are poorly understood. In this study, PnATX1 and PnATX2 of Populus simonii × P. nigra were identified and characterized. Sequence analysis showed that PnATXs contained the metal-binding motif MXCXXC in the N-terminus and a lysine-rich region. Phylogenetic analysis of ATX protein sequences revealed that PnATXs were clustered in the same group as AtATX1. PnATX proteins were localized in the cytoplasm and nucleus. Tissue-specific expression analysis showed that PnATX1 and PnATX2 were expressed in all analyzed tissues and, in particular, expressed to a higher relative expression level in young leaves. Quantitative real-time PCR analysis indicated that each PnATX gene was differentially expressed in different tissues under treatments with copper, zinc, iron, jasmonate and salicylic acid (SA). The copper-response element GTAC, methyl jasmonate and salicylic acid responsiveness elements and other cis-acting elements were identified in the PnATX1 and PnATX2 promoters. Expression of β-glucuronidase driven by the PnATX1 promoter was observed in the apical meristem of 7-day-old Arabidopsis transgenic seedlings, and the signal strength was not influenced by deficient or excessive copper conditions. Both PnATX1 and PnATX2 functionally rescued the defective phenotypes of yeast atx1Δ and sod1Δ strains. Under copper excess and deficiency conditions, transgenic Arabidopsis atx1 mutants harboring 35S::PnATX constructs exhibited root length and fresh weight similar to those of the wild type and higher than those of Arabidopsis atx1 mutants. Superoxide dismutase activity decreased in transgenic lines compared with that of atx1 mutants, whereas peroxidase and catalase activities increased significantly under excess copper. The results provide a basis for elucidating the role of Populus PnATX genes in copper homeostasis.</AbstractText><CopyrightInformation>© The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Zhiru</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Life Science, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Jiahuan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Life Science, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qu</LastName><ForeName>Chunpu</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Ruhui</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>College of Life Science, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Jinyuan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Life Science, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Qi</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xi</LastName><ForeName>Qi</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Yang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Life Science, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Qi</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Chuanping</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Guanjun</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Forestry, Northeast Forestry University, Harbin, 150040, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">ATX1-type protein</Keyword><Keyword MajorTopicYN="Y">copper stress</Keyword><Keyword MajorTopicYN="Y">functional complementation</Keyword><Keyword MajorTopicYN="Y">promoter analysis</Keyword><Keyword MajorTopicYN="Y">transgenic line</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>10</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>12</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>01</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>2</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>2</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32031640</ArticleId><ArticleId IdType="pii">5728676</ArticleId><ArticleId IdType="doi">10.1093/treephys/tpaa008</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Xu, Zhiru" sort="Xu, Zhiru" uniqKey="Xu Z" first="Zhiru" last="Xu">Zhiru Xu</name></noRegion><name sortKey="Chang, Ruhui" sort="Chang, Ruhui" uniqKey="Chang R" first="Ruhui" last="Chang">Ruhui Chang</name><name sortKey="Chen, Jinyuan" sort="Chen, Jinyuan" uniqKey="Chen J" first="Jinyuan" last="Chen">Jinyuan Chen</name><name sortKey="Huang, Jiahuan" sort="Huang, Jiahuan" uniqKey="Huang J" first="Jiahuan" last="Huang">Jiahuan Huang</name><name sortKey="Liu, Guanjun" sort="Liu, Guanjun" uniqKey="Liu G" first="Guanjun" last="Liu">Guanjun Liu</name><name sortKey="Liu, Guanjun" sort="Liu, Guanjun" uniqKey="Liu G" first="Guanjun" last="Liu">Guanjun Liu</name><name sortKey="Qu, Chunpu" sort="Qu, Chunpu" uniqKey="Qu C" first="Chunpu" last="Qu">Chunpu Qu</name><name sortKey="Qu, Chunpu" sort="Qu, Chunpu" uniqKey="Qu C" first="Chunpu" last="Qu">Chunpu Qu</name><name sortKey="Song, Yang" sort="Song, Yang" uniqKey="Song Y" first="Yang" last="Song">Yang Song</name><name sortKey="Sun, Qi" sort="Sun, Qi" uniqKey="Sun Q" first="Qi" last="Sun">Qi Sun</name><name sortKey="Sun, Qi" sort="Sun, Qi" uniqKey="Sun Q" first="Qi" last="Sun">Qi Sun</name><name sortKey="Wang, Qi" sort="Wang, Qi" uniqKey="Wang Q" first="Qi" last="Wang">Qi Wang</name><name sortKey="Wang, Qi" sort="Wang, Qi" uniqKey="Wang Q" first="Qi" last="Wang">Qi Wang</name><name sortKey="Xi, Qi" sort="Xi, Qi" uniqKey="Xi Q" first="Qi" last="Xi">Qi Xi</name><name sortKey="Xi, Qi" sort="Xi, Qi" uniqKey="Xi Q" first="Qi" last="Xi">Qi Xi</name><name sortKey="Xu, Zhiru" sort="Xu, Zhiru" uniqKey="Xu Z" first="Zhiru" last="Xu">Zhiru Xu</name><name sortKey="Xu, Zhiru" sort="Xu, Zhiru" uniqKey="Xu Z" first="Zhiru" last="Xu">Zhiru Xu</name><name sortKey="Yang, Chuanping" sort="Yang, Chuanping" uniqKey="Yang C" first="Chuanping" last="Yang">Chuanping Yang</name><name sortKey="Yang, Chuanping" sort="Yang, Chuanping" uniqKey="Yang C" first="Chuanping" last="Yang">Chuanping Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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